黄秋葵水提多糖工艺优化

文档编号:HG058  文档字数:9549,页数:22

摘 要：本文档以浙江衢州产黄秋葵嫩荚果为原料，首先将其加工成冻干粉，然后利用水提的方法，经由硫酸苯酚法来测取离心试样溶液的吸光度，以此来测定黄秋葵冻干粉中的多糖含量。首先开展单因素试验，分别通过对水提温度、水提时间和料水比三个因素的比较，获得各因素对多糖提取含量的影响。再设计正交试验，改变水提时间、水提温度和料水比三个变量，以获得水提黄秋葵多糖的最佳试验条件。研究得到的水法提取黄秋葵多糖的最佳试验条件为：水提时间4小时，水提温度50℃，料水比（冻干粉：蒸馏水）1：20。另根据试验得知，水提次数越多，黄秋葵多糖的得率越高。
 
关键词：黄秋葵；多糖；水提；正交

Optimization of Okra Polysaccharide by water extraction
Abstract: This article use Okra fruit pods as raw materials, processed into freeze-dried powder，using water extraction methods, by phenol sulfuric acid method for measurement of the absorbance of the sample solution was centrifuged, in order to determine okra polysaccharide content of freeze-dried powder. First of all, to carry out single factor experiments were provided by the water temperature, extraction time and ratio of water extraction of three factors are the impact on the polysaccharide. Then use orthogonal experimental method, by changing the extraction time, water extraction temperature, and the ratio to get the water extract the best experimental conditions for okra polysaccharide. Of water extraction by okra polysaccharide optimal experimental conditions: water extraction time of 4 hours, water extraction temperature 50℃, the ratio of water (freeze-dried powder: water) 1:20. According to experimental results, the more the number of water extraction, and the higher the yield of okra polysaccharide.

Keywords: Okra; polysaccharide; water extraction; orthogonal.
 

目  录

中文摘要 I
英文摘要 II
目录 III
1 绪论 1
1.1  前言 1
1.2  黄秋葵与多糖 1
1.2.1  黄秋葵基本情况 1
1.2.2 多糖的性质 2
1.2.3 生物多糖的提取方法 3
2 试验部分 6
2.1  主要试验试剂与仪器 6
2.1.1 试验试剂 6
2.1.2 试验仪器 6
2.2  试验方法 6
3  结果与讨论 11
3.1  单因素试验结果与讨论 11
 3.2  正交试验结果与讨论 13
4 结论与展望 15
4.1 试验结论 15
4.2 展望 15
致 谢 17
参考文献 1

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