正交试验超声优化茯苓多糖提取工艺

文档编号:HG104  文档字数:13305,页数:30

摘 要：在单因素实验的基础上利用正交实验设计考察碱法提取过程中碱浓度、提取时间、料液比对茯苓多糖提取率的影响，确定最佳提取工艺条件为 : NaOH浓度 0. 75mo l /L ,时间2min ,料液比 1∶40。在此工艺条件下茯苓多糖的提取率高达86%。
关键词：茯苓多糖；超声波；提取；正交试验
 Study on the Extraction Technology of  Poria cocos  Polysaccharide under Ultrasound by Orthogonal Design

Abstract: With the assistance of ultrasound, the polysaccharide from Poria cocos wolf was extracted using NaOHaq. The influence of extraction conditions including extraction time, ratio of raw materials and NaOHaq(V), concentration of media were studied. Then the optimization of conditions of extraction by the orthogonal design was investigated, it was found that the optimum conditions were NaOH 0.75mol/L, extraction time 2min, materials/solution1:40. Under the best condition, the extraction ratio of polysaccharide from Poria cocos wolf was as high as 86%.
Key words： Poria cocos polysaccharide ; ultrasonic wave ; extraction ; orthogonal test

 
 
 目  录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1 茯苓的概述 1
 1.2  茯苓多糖的概述 2
 1.3  茯苓多糖衍生物的作用 4
 1.4  超声波的基本作用原理及在提取生物活性物中的作用 5
 1.5 稀碱浸提法 6
 1.6 研究思路 7
2. 实验部分 8
 2.1 实验原料、试剂及试验仪器 8
     2.2 实验原理 8
 2.3 实验方法 9
3. 结果与讨论 12
 3.1 波长的选择 12
 3.2 标准曲线的制作 12
 3.3 换算因子的确定 13
  3.4 单因素分析 13
  3.5 提取工艺正交优化实验 17
 3.6 茯苓多糖的红外光谱分析 19
4. 总结与展望 21
     4.1结论 21
 4.2存在的问题 22
 4.3展望 22
致谢 23
参考文献 24

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