正交优化菜籽油甾醇提取工艺

文档编号:HG133  文档字数:8177,页数:22

 摘 要：对植物甾醇提取工艺进行优化，得出较佳提取条件，可应用于植物甾醇的提取。以10mL菜籽油的植物甾醇产量为指标，对萃取剂用量、提取过程中反应温度，萃取的次数，液料比，反应时间等因素进行了单因素考察，最后用正交法进行优化。实验结果表明：萃取剂用量60mL，反应温度93℃，提取次数1次，取时间为45min，萃取剂用量50mL, 液料比为5:1；溶剂回收再利用可行。通过正交设计得出较佳条件为：提取不皂化物时，加氢氧化钠-乙醇溶液40mL，在93℃下皂化60min，再用110mL乙醚萃取1次。
 关键词：菜籽油；甾醇；提取；正交优化

Abstract: In this article the optimization experiment of the extraction of phytosterols from rapeseed crude oil was carried out and the optimal extraction condition was determined, and accordingly to be used in industrialization extracting of phytosterols. In the experiment extraction yield of phytosterol from 10mL rapeseed crude oil was taken as an index, extraction agent, extraction temperature, extraction times, solid-liquid ratio, and extraction time in the extraction process were studied, finally the experiment was optimized by orthogonal. The results showed that under the solid-liquid ratio was 5:1(V/V), the time of extraction was 45 min, the extractant volume was 50 mL，the temperature of extraction was 93℃, the extractant tim was 1, liquid ratio was 5:1; The solvent could be recycled. Obtained by orthogonal design, the better conditions were obtained by orthogonal design as follows: when extracted unsaponifiable matter, adding sodium hydroxide-ethanol 40mL, saponification at 93℃ under 60min, then 110mL ether extraction 1 time.
Keywords：Rapeseed crude oil；Phytosterols；Extraction；Orthogonal optimization

目  录
中文摘要 I
英文摘要 II
目录 III
1  绪论 1
 1.1  植物甾醇的结构和理化性质 1
 1.2  关于提取植物甾醇的研究 2
 1.3  植物甾醇应用 3
 1.4  植物甾醇的开发及研究现状 5
2  实验部分 6
 2.1  实验原料，试剂与仪器 6
 2.2  实验步骤 6
3  结果与讨论 8
 3.1  单因素考察 8
 3.2  正交实验结果及极差分析 11
 3.3  溶剂的回收 13
 3.4  对比实验 14
4  总结与展望 15
致谢 16
参考文献 17

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