高产SOD菌株HB25的发酵条件优化研究

文档编号:SW048  文档字数:11143,页数:22

摘  要：采用正交试验及单因子条件实验对菌株HB25进行发酵产酶的影响因素及发酵工艺条件进行了研究。结果表明：其最佳培养基组成为葡萄糖2%，蛋白胨2%，酵母膏1%，7Be麦芽汁9%（V/V），CuSO475mmol/ml，ZnSO425mmol/ml ；最佳培养条件为：初始pH5.5,摇瓶装量为75 ml/500 ml三角瓶，接种量为15%，28C培养20小时，加H2O2使终浓度为1%，继续培养至24小时。在此条件下，该菌细胞生物量为5.26g/100ml，SOD含量为2662u/g湿菌体，SOD产量为 1.40104u/100ml，SOD产量较出发菌株提高99%。
关键词：菌株HB25；发酵条件；优化

Studies on Optimizing fermentation condition of strain HB25

Abstract: Based on orthogonal and monofactorial test, the medium and fermentation condition was studied. It showed the optimal medium consisted of 2% glucose, 9%(V/V) malt wort, 2%peptone, 1%yeast extract, CuSO475mmol/ml, ZnSO4 25mmol/ml; the optimal culture condition were as following: Initial pH 5.5, medium cubage was 75ml/500ml, 15% concentration was inoculated; After 20 hours, H2O2  was added to the 1% final concentration, then cultured to 24 hours. Under this condition, the biomass was 5.26g/100ml, SOD activity was 2662u/g fresh cell, SOD production was 1.40104 u/100ml, which was 99% higher than original strain.

Key words: Strain HB25; Fermention condition; Optimization

目  录
中文摘要 I
英文摘要. II
目  录 Ⅲ
1．绪论 1
1.1 SOD的种类及分布 1
1.2 SOD的催化机理及活性中心 2
1.3 SOD的主要应用 3
1.4 国内外研究概况 5
1.5 本项目的研究目的及意义 6
2．实验部分 7
2.1 材料与方法 7
2.2 试验设计 8
3．结果与讨论 10
3.1 发酵工艺条件实验结果 10
3.2 培养基组成单因子条件试验结果 11
3.3 优化培养条件的结果 13
3.4 小结 13
4．结论与展望 14
致谢 15
参考文献 16

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