|
文档编号:SW098 文档字数:10174,页数:30 摘要：羊肚菌是一种十分重要的食药兼用真菌，含有丰富的生理活性物质，多糖是其中的重要一种，具有增强肌体免疫力、抗疲劳、抗病毒、抑制肿瘤等多种功能。本实验羊肚菌液体发酵最适发酵温度为25℃，最适的转速为150rpm；溶解氧会抑制菌丝体的生物量和多糖和积累；分离菌丝体和发酵液，然后采用超声破壁，1:40的浸提比浸提3h，浸提次数为两次，用95%的乙醇醇析2d，再用无水乙醇洗涤两次、Sevage法脱蛋白后用ΦMW3500 透析制取胞内和胞外多糖；采用苯酚-硫酸法测定胞内多糖的总糖，最高的得率有3.5%；液相分析结果表明，胞内还有游离的单糖，胞外则不含游离的单糖；用2mol/L的硫酸水解后的多糖进行液相分析，结果表明，羊肚菌多糖可能有葡萄糖、半乳糖、甘露糖、木糖。 Deep fermentation fo Morchella and extraction of polysaccharide Abstract: Morchella is a very important edible and medicinal fungi, is rich in physiologically active substances, polysaccharide is one of the important one, with enhanced body immunity, anti-fatigue, anti-virus, and many other features of tumor. In this experiment, the optimum fermentation fermented morel liquid temperature was 25 ℃, the optimal speed is 150rpm; Dissolved oxygen will inhibit the mycelium biomass and polysaccharide and accumulation; Separation of mycelium and fermentation broth, and then broken using ultrasonic, 1:40 extraction of ratio, extraction 3h, extraction times for the two, with 95% ethanol precipitation 2d, then using ethanol washing twice and suing Sevage removal protein , dialysis with ΦMW3500 ,the and Receive the polysaccharide. Liquid chromatography analysis showed that there are free intracellular monosaccharide, is non-free extracellular monosaccharide; With 2mol / L sulfuric acid hydrolysis of the polysaccharide Liquid chromatography analysis, the results show that the polysaccharides may be glucose, galactose, mannose, xylose. 目录
|
|
| |
| 上一篇：酸奶中风味物质的含量检测 | 下一篇：油菜转录因子BnWRI1提高种子含油.. |
| 推荐论文 | 本专业最新论文 |
| Tags：羊肚菌 深层 发酵 多糖 提取 分析 | 2011-03-31 16:41:38【返回顶部】 |
