响应面法优化两相体系发酵生产天然香料β-苯乙醇

文档编号:SW157  文档字数:12590,页数:30

摘 要：考查了马克斯克鲁维酵母 AS2.1440 在有机溶剂/水两相系统中生物转化L-苯丙氨酸生产 β-苯乙醇的过程。在单因素试验的基础上，用Plackett-Burman 设计、最陡爬坡试验结合响应面分析法对影响β-苯乙醇的因素进行了研究，确定了最佳发酵条件为：两相比例控制在油酸/水=2 : 3为宜；装液量为64.46 mL/150mL三角瓶，培养基初始pH控制在5.0，温度32.99℃，转速160 r/min下转化48 h，β-苯乙醇的产量可达1533±11 mg/L。与传统水相相比，油酸-水两相系统中Kluyveromyces marxianus AS2.1440 转化 L-苯丙氨酸生产 β-苯乙醇的产量提高了 102.91%。

关键词：两相体系；β-苯乙醇；响应面方法； AS2.1440

Abstract: Bioconversion of L-phenylalanine to β-phenylethylalcohol with Kluyveromyces marxianus AS2.1440 was performed in an aqueous/organic solvent biphasic system. On the basis of single-factor tests, factors affecting Bioconversion of L-phenylalanine to β-phenylethylalcohol with Kluyveromyces marxianus AS2.1440 were evaluated by Plackett-Burman design, the path of steepest ascent and response surface methodology. The optimal bioconversion conditions are as follows: the concentration of L-phenylalanine was 4 g/L, the optimum values of volume ratio of organic phase to aqueous phase, reaction temperature, pH value and liquid volume were 2 : 3, 32.99℃, 5.0, and 64.46mL/250mL respectively. Under above optimum conditions, the content of β-phenylethylalcohol reached1533±11g/L48 h later, which increased by about 102.91% than that in monophasic aqueous system.

eywords：biphasic system; β-phenylethylalcohol; Response Surface Methodology; Kluyveromyces marxianus AS2.1440

目  录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1  β-苯乙醇的性质和用途 1
 1.2  β-苯乙醇的生产方法 1
 1.3  两相体系 5
 1.4  市场前景 5
2. 实验部分 6
 2.1  实验材料 6
 2.2  实验方法 7
 2.3  实验设计方法 9
3. 结果与讨论 16
 3.1  有机溶剂的选择 17
 3.2  水/有机溶剂两相体系与水相体系的反应过程比较 17
 3.3  Plackett-Burman 设计结果与讨论 18
 3.4  最陡爬坡实验 20
 3.5  响应面方法优化发酵条件 21
 3.6  验证试验 23
4．总结与展望 23
致谢 24
参考文献 25

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