TNF-α单克隆抗体Fd链基因的分子克隆和表达

文档编号:SW230  文档字数:10439,页数:17

摘 要：肿瘤坏死因子是一种促炎性细胞因子，在免疫反应、炎症和对损伤反应中起重要作用，主要影响细胞增殖和细胞凋亡的调节，不仅对肿瘤有细胞毒、细胞溶解、抑制增殖、诱导细胞凋亡等作用，还影响多种正常细胞的生长分化，并与其他细胞因子一起形成复杂的免疫网络。
 本文应用分子生物学PCR扩增技术、PCR产物纯化及连接插入载体（pUC）技术、制备感受态细胞技术、外源DNA转化技术、质粒提取技术、DNA测序技术、原核表达技术等开发抗TNF-α Fab小分子抗体药物，治疗与TNF-α作用相关的疾病。
关键词：PCR扩增技术；基因克隆；大肠杆菌；Fd基因；重组质粒

Abstract：TNF-α（Tumor necrosis f factor-alpha）is a proinflammatory cytokine, the immune response, inflammation and injury response play an important role, mainly affect cell proliferation and apoptosis of cells, not only to tumor cytotoxicity, cell lysis, inhibition of proliferation and induction of apoptosis, etc, but also a variety of normal cell growth and differentiation with other cytokines together to form a complex immune network.
 Using molecular biology PCR, PCR products were purified and inserted connecting carrier (gel) technology, Preparation of competent cell technology, foreign DNA into technology, plasmid extraction, DNA sequencing technology, prokaryotic expression technology, we development anti-TNF-Europium antibody Fab small molecule drugs for the treatment of TNF-Europium role related diseases.
 Keywords : Amplified by PCR technology; Gene cloning; E.coli; Fd gene; Recombinant plasmid

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