分光光度法测定氨基酸型肠内营养剂的胆碱含量

文档编号:SW232  文档字数:9273,页数:25

分光光度法测定氨基酸型肠内营养剂的胆碱含量
摘  要
 采用分光光度法，在波长500 nm处测定氨基酸型肠内营养剂中的胆碱吸光度。结果表明，在2.5～12.7μg/mL浓度范围内，吸光度与浓度呈良好的线性关系（r = 0.9997），加标回收率为95.33-104.05%（RSD = 2.8%），方法具有良好的重现性和稳定性，采用该法对日本新引进的氨基酸型肠内营养剂的胆碱含量进行了测定。

关键词：氨基酸型肠内营养剂 胆碱 分光光度法 含量测定

Determination of choline in Enternal Nutrition,Amino Acid,
 Elental by spectrophotometry
ABSTRACT
 In this paper, the absorbance of choline in Enternal Nutrition,Amino Acid, Elental solution was measured by spectrophotometry at the wavelength 500 nm. The results showed that there was a good linear relationship (r = 0.9997) between the absorbance and concentration at the range of 2.5～12.7μg/ml, and the recovery was at the range of 95.33-104.05%（RSD =2.8%）. This method was approved to have good reproducibility and stability which was applied to measure the phosphatidylcholine content in Enternal Nutrition,Amino Acid, Elental which was recommended from Japan.

Key words: Enternal Nutrition,Amino Acid  Elental; lcholine  Spectrophotometry
 Content determination
 

目  录
      
 摘  要 I
 ABSTRACT II
 1前言 1
 1.1 肠内营养制剂的简介 1
 1.2胆碱简介 2
 1.2.1胆碱生理功能，见图1-2 2
 1.2.2胆碱含量对人体健康的影响 2
 1.3检测胆碱的目的与意义 3
 1.4不同测定方法的比较与最终选定 4
 2实验部分 6
 2.1 仪器及试药 6
 2.1.1 仪器 6
 2.1.2试剂 6
 2.2实验方法 6
 2.2.1胆碱的定量方法 6
 2.2.1.1原试验方法[15] 7
 2.2.1.2现拟定方法 7
 3结果与讨论 8
 3.1方法学验证 8
 3.1.1标准曲线的绘制 8
 3.1.2重复性及稳定性 9
 3.1.3显色时间 10
 3.1.6样品含量测定 12
 3.2.试验方法的变更点及其理由 12
 3.2.1供试品溶液制备方法的变更 12
 3.2.2对照品标准溶液制备方法的变更 13
 3.2.3测定条件的变更 14
 3.3总结 14
 3.3.1样品性质 14
 3.3.2微量含量 14
 3.3.3质量控制 14
 3.3.4温度控制 15
 4小结 16
 附录 18
 1.标准曲线制备设计图 18
 2.回收率计算过程 19
 3.公式 20
 谢辞 21

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