鲨鱼软骨中提取硫酸软骨素的工艺比较

文档编号:ZY115    文档字数:11323,页数:24

摘 要：随着空气、水质、土壤等环境因素的日益恶化，人类身体健康受到巨大的危害。当今世界人类死亡的头号杀手——癌症，已在我们的生活中时常见到。研发抗癌药物是当今医学界的一项重要工作。研究发现鲨鱼软骨中提取的硫酸软骨素，对许多疾病有很好疗效，有很高的开发利用价值．因此，如何提高鲨鱼硫酸软骨素工业化生产中的产品得率、降低生产成本，已引起工业界的广泛重视。
 本文选用碱解-双酶解相结合的方法，考察了温度、、双酶加量等对提取物中含氮量的影响。结果表明：温度为，值，双酶加量的条件下得到的硫酸软骨素含氮量最低。该工艺反应条件温和，污染环境小，便于推广，具有工业化批量生产的实用价值。
关键词:硫酸软骨素;提取; 工艺比较

Abstract:With the increasing deterioration of environmental factors such as air, water and oil, human health hazards are enormous. The world's first killer to human death – cancer can be often seen in our life and reasearch and development on anticancer drugs has been an important task in medical field. The foundation that chondroitin sulfate extracted from shark cartilage has a good efficacy against many diseases, which have high economic value, make the manner of how to improve industrial production of shark chondroitin sulfate and reduce production costs in the product yield cause wide attention in industry.
 Through the combined method of Alkaline Hydrolysis and both enzymatic hydrolysis, the influence on extract’s nitrogen content of temperature,  and pancreas enzyme increment can be detected. It is resulted that the lowest nitrogen content of chondroitin sulfate can be reached with the temprature of ,  of  and pancreas enzyme increment of . This craft has mild response condition and little pollution, besides, it is easy to be pomoted. All of this make it have practical value in industrialized mass production.

Keywords：Sulfuric acid chondroitin; Extraction; Process Comparison

目  录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1  硫酸软骨素的现阶段研究状况 1
 1.1.1  硫酸软骨素的物理性质 1
 1.1.2  硫酸软骨素的化学性质 2
 1.1.3  硫酸软骨素的生物活性 3
 1.2  硫酸软骨素的药理作用 3 
 1.2.1  抗关节炎作用 3
 1.2.2  免疫抑制作用 3
 1.2.3  调脂、降脂和抗动脉粥样硬化作用 4
 1.2.4  抗凝作用 4
 1.2.5  神经元的保护和修复作用 4
 1.3  硫酸软骨素的应用 4
 1.3.1  医学 4
 1.3.2  食品 5
 1.3.3  化妆品 5
 1.4  硫酸软骨素的提取和检测 5
 1.4.1  硫酸软骨素的提取 5
 1.4.2  硫酸软骨素的检测 9
2. 实验部分 10
 2.1  实验试剂与仪器 10
 2.2  原理预处理 11
 2.3  含氮量分析 12
3. 结果与讨论 13

 3.1   影响酶解反应的因素 13
 3.1.1  反应温度的影响 13
 3.1.2  反应pH值的影响 14
 3.1.3  双酶加量的影响 15
 3.2  双酶解条件的优化 15
4．总结与展望 16
 4.1  结论 16
 4.2  展望 17
致谢 18
参考文献 19

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