枯草芽孢杆菌微生态制剂的研制

文档字数:10478,页数:21

枯草芽孢杆菌微生态制剂的制作
摘要：目的 提高枯草芽孢杆菌微生态制剂的活菌数以及芽孢率。方法 采用单因素实验确定枯草芽孢杆菌的最佳的发酵条件以及通过枯草芽孢杆菌加工中的两种干燥方式的比较来确定最佳干燥方式。结果 最适发酵条件为：温度37℃，pH值7.0，装量20ml/250ml锥形瓶，转速195r·min-1，枯草芽孢杆菌在真空冷冻干燥和真空干燥的条件下制剂的最高活菌数分别可达1.1×109cfu/g和0.7×109cfu/g，最高存活率为86.62%和71.51%。结论 优化培养基发酵条件使最终活菌数提高了约17.5 %，芽孢形成比率高，菌体死亡率较优化前降低24.7 %。

关键词: 枯草芽孢杆菌；干燥条件；培养条件；优化

The production of Bacillus subtilis probiotics
Abstract ：Objective  Optimize the production parameters of Bacillus subtilis probiotics and the conditions of Bacillus subtilis fermentation. Methods  Using single factor determining Bacillus subtilis best fermentation conditions and the adoption of Bacillus subtilis processing of two dry dry way of comparison to determine the best drying methods.  Results  optimum fermentation conditions : temperature 37℃, pH 7.0, load 20 ml/250ml tapered bottles, speed 195 r ·min-1. Bacillus subtilis in a vacuum freeze-drying and vacuum drying agents under the conditions of the maximum number of living bacteria were up 1.1×109CFU / g and 0.7 ×109CFU/ g, the highest survival rate was 86.62% and 71.51%. Conclusion  Optimal conditions for the fermentation medium ultimately counted increased by about 35.2%, the ratio of high spore formation, Optimization of cell mortality decreased over 24.7%.

Keywords:  Bacillus subtilis；dry conditions；cultural condition；optimization

目录
中文摘要 I
英文摘要 Ⅱ
目录 Ⅲ
1．绪论 1
2．实验部分 5
 2.1材料和仪器 5
 2.1.1材料 5
 2.1.2仪器 5
 2.2方法 5
     2.2.1培养基的配制 5
 2.2.2溶液的配制 5
     2.2.3检测方法 5
 2.2.4菌种的筛选 6
 2.2.5发酵条件的优化 6
 2.2.6干燥条件的优化 7
 2.2.7生长曲线测定 7
3．结果与讨论 8
 3.1菌种的优化 8
 3.2培养条件的优化 9
 3.2.1温度 9
 3.2.2起始pH值 10
 3.2.3装量 10
 3.2.4接种量 11
 3.3细菌生长曲线测定 11
 3.4真空干燥温度筛选 12
 3.5两种干燥方式对比 13
4．总结与展望 14
致谢 15
参考文献 16

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