乌贼内壳中β-壳聚糖的制备研究

文档编号:SW015  文档字数:10660,页数:22

摘　要:选用乌贼内壳为原料，用化学方法提取出β-甲壳素，研究了将β-甲壳素进行脱乙酰化制备β-壳聚糖的最优化设计。实验考察氢氧化钠浓度、反应时间、反应温度各个因素对β-壳聚糖脱乙酰度的影响，选择了三因素三水平的正交实验方案L9(33)。对实验数据图表进行分析，得出各个因素对于β-壳聚糖脱乙酰度的影响程度各不相同，其中氢氧化钠的浓度对于实验结果的影响最显著，反应温度次之，反应时间影响最小。结果表明：β-壳聚糖的脱乙酰度随着氢氧化钠溶液的浓度、反应温度和反应时间的增加而增大。对正交实验结果进行分析处理后，得出甲壳素脱乙酰的最优条件为氢氧化钠的浓度为60%，反应温度为110℃，反应时间为4小时。在该反应条件下得到β-壳聚糖的脱乙酰度为85.92%。
关键词: 乌贼；β-甲壳素；β-壳聚糖；脱乙酰

Study on the Preparation of β-Chitosan of Sepia Shell
Abstract: Sepia shell was chosen as the raw material for the extraction of β-chitin. The optimal design of the deacetylation conditions of β-chitin which used to prepare β-chitosan was studied in this paper . The influence of the deacetylation temperature, deacetylation time, and sodium hydroxide concentration on the degree of deacetylation(DD) of deacetylated products prepared from β-chitin were discussed . A L9 (33) orthogonal table was used to complete the preparation of β-Chitosan . The results revealed that the DD values of β-Chitosan increase as the deacetylation temperature, deacetylation time, and sodium hydroxide concentration increases. Among these three factors, the sodium hydroxide concentration influenced the results significantly, and the second was the deacetylation temperature. The result showed that the most suitable and proper reaction conditions of deacetylation was that: the concentration of sodium hydroxide was 60%, the deacetylation temperature was 110℃, the deacetylation time was 4 hours, and the DD of the product was 85.92%.
Key words : Sepia; β-Chitin; β-Chitosan; Deacetylation

目  录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1  前言 1
      1.2  文献综述 2
2. 实验部分 7
 2.1 实验原理  7
 2.2 实验原料与仪器  7
 2.3 实验步骤 8
 2.4 实验工艺流程 10
3. 结果与讨论 11
 3.1 单因素实验   11
 3.2 正交实验 12
 3.3 实验结果数据处理 13
 3.4 产物的红外分析 14
4. 总结与展望 16
致谢 17
参考文献 18

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