IPTG诱导半乳糖苷酶基因在大肠杆菌中表达的研究

文档编号:SW017  文档字数:7316,页数:19

 摘 要：β-半乳糖苷酶是大肠杆菌内的内源性酶，是由lac Z基因转录而来的。这种lac Z基因是一种广泛使用的报告基因，这种基因启动子的融合在不同情况下被用于研究各种转录和表达。本实验主要通过在大肠杆菌培养液中添加IPTG，去诱导表达β-半乳糖苷酶，并测试其酶的活性并估算诱导的效果。在加入IPTG诱导10分钟内，细胞中β-半乳糖苷酶的合成并没有显著增加；当诱导反应时间经过40分钟后，β-半乳糖苷酶合成显著上升。

关键词：大肠杆菌；半乳糖苷酶；诱导

The Induction of β-galactosidase in Escherichia coli

Abstract: β-galactosidase is an endogenous enzyme in wild type E.coli, and it is transcribed from the lacZ gene. The lacZ gene is a widely used reporter gene and these promoter fusions have been used in the study of various transcription and expression of genes in different conditions by quantifying β-galactosidase activity. This study mainly through the medium of E. coli added IPTG, to induce expression of β-galactosidase enzyme and test their estimates of the activity and induced effects. The β-galactosidase enzyme synthesis of cells increased not significanthy in 10 minutes, After IPTG was join into the culture; However The β-galactosidase enzyme synthesis increased signifiamthy after 40 minutes.

Keywords：E.coli; galactosidase; Induction

目  录
中文摘要 II
英文摘要 III
目录 IV
1. 绪论 1
 1.1 大肠杆菌 1
 1.2 半乳糖苷酶 2
 1.3 实验目的 4
2. 实验部分 5
 2.1 实验器材 5
 2.2 实验试剂 5
 2.3 实验步骤 5
3. 结果与讨论 7
 3.1 数据处理的方法 7
 3.2 结果分析 8
 3.3 讨论 11
4.总结与展望 13
致谢 14
参考文献 15
附录 16

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