Fe（Ⅱ）EDTA-NO高效还原菌特性研究

文档编号:SW082  文档字数:12523,页数:30

摘 要：本文采用了络合吸收结合生物转化的方法，将化学吸收，生物还原法两种方法结合在一起，首先通过络合吸收剂的作用，使NO从气相转入液相，然后在微生物的作用下将其还原为氮气，充分利用两者优势使其缺陷得以互补。在恒温摇床中对微生物还原NO络合吸收液的特性进行了研究。
 实验结果证明：不同浓度的葡萄糖、Fe(Ⅱ)EDTA-NO、Fe(Ⅲ)EDTA和NH4+ 对DN-3菌还原络合物Fe(Ⅱ)EDTA-NO能力及其生长有影响。随着Fe(Ⅱ)EDTA-NO浓度的上升，细菌对其的还原速率先上升后下降，期间达到一个最大比还原速率。加入一定浓度的氯化铵能够提高Fe(Ⅱ)EDTA-NO的还原速率，但是还原速率增长并不显著。在外加少量适宜碳源的条件下，就可以实现络合吸收剂的不断循环利用。最后鉴定菌种，鉴定结果DN-3菌归属为肠杆菌属中的阴沟肠杆菌(Enterobacter cloacae)。
关键词：络合吸收；生物还原；细菌分离；鉴定菌种

Fe (Ⅱ) EDTA-NO highly effective return to original state germ characteristic research

Abstract: This article has used the complexing absorption union biotransformation method, The chemical absorption - biology reducing process two methods unifies in together, Through complexing absorbent function, Enable NO to change over to the liquid phase from the gas phase, Then returns to original state under microorganism's function it into the nitrogen, Uses both superiority to enable its flaw fully supplementary. Returned to original state the NO complexing absorption solution characteristic in the constant temperature table to the microorganism to conduct the research.
 Experimental result proof: Different density glucose、Fe(Ⅱ)EDTA-NO、Fe(Ⅲ)EDTA and NH4+ to DN-3 fungus return to original state complex compound Fe(Ⅱ)EDTA-NO The EDTA-NO ability and grows influential. Along with Fe(Ⅱ)EDTA-NO density rise, The bacterium drops after its return to original state fast lead rise, Period achieves a maximum ratio return to original state speed. Joins certain density the ammonium chloride to be able to enhance Fe(Ⅱ)EDTA-NO EDTA-NO return to original state speed, But the return to original state speed grows is not remarkable. In under sur- few suitable carbon source condition, May realize the complexing absorbent unceasing circulation use. Finally appraises the mold mushroom spawn, Appraisal result DN-3 fungus ownership for intestines pole bacteria's in sewer intestines bacillus (Enterobacter cloacae).

Key word: Complexing absorption; Biological return to original state;Bacterium separation;appraises the mold bacterium

目 录
中文摘要 I
英文摘要 II
目 录 III
1. 绪 论 1
1.1  空气的重要性 1
1.2  NOx的来源及危害 1
1.3  目前的主要治理方法 3
1.4  立题依据及研究内容 4
1.5 小结 5
2. 实验部分 7
2.1  驯化微生物还原NO络合吸收液 7
3. 结果与讨论 13
3.1  菌种鉴定 13
3.2  氯化铵对于细胞生长和Fe(Ⅱ)EDTA-NO还原效果的影响 13
3.3  不同初始浓度Fe(Ⅱ)EDAT-NO对于还原效果的影响 14
3.4  以Fe(Ⅱ)EDTA和葡萄糖作为电子供体还原Fe(Ⅱ)EDTA-NO时中间产物的测定结果分析 15
3.5  小结 16
4.结论与展望 18
4.1  结论 18
4.2  展望 18
致  谢 19
参考文献 20
附录 A 22
附录 B 24
附录 C 25

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