固定化细胞法生产醋酸的优化研究

文档编号:SW112  文档字数:14869,页数:27

摘  要：本文是以海藻酸钠为载体，固定化醋酸菌发酵乙醇生产醋酸。研究了固定化细胞的制备，制备的颗粒的半径和体积大小。另外，还对接种环数、种子培养时间进行了优化。最主要的是研究了不同酒精初始浓度对乙酸产量、菌体个数、菌体生长周期、葡萄糖消耗量、乙醇消耗量的影响。最后我们得到如下结论:（1）固定化细胞颗粒平均直径为5.0mm，颗粒体积为65.42mm3；（2）优化接种数在15环、培养时间为36-48小时之间，发酵液菌体个数最多；（3）葡萄糖浓度与葡萄糖OD值的换算公式：y=4.7237x，单位为mg/ml；（4）菌体个数与菌液OD值的相关性：y = 402.88x，单位为107个/毫升；（5）酒精初始浓度对乙酸产量的影响：在各酒精浓度中，5%酒精浓度的发酵液乙酸转化率最高，最佳培养时间为96小时；（6）酒精初始浓度对菌体个数的影响：不同的酒精初始浓度影响着菌体的生长，酒精浓度越高对菌体的生长起着延长生长延迟期的作用；（7）酒精初始浓度对葡萄糖消耗的影响：乙醇的含量越高则葡萄糖的消耗就越少；（8）酒精初始浓度对乙醇消耗的影响：在72小时时间点，乙醇消耗完毕后，醋酸在72-96小时时间段的继续生成，是由于乙醛没随乙醇的消耗完毕马上转化为醋酸而引起的滞后反应。

关键词：固定化；乙醇；醋酸菌；醋酸钙镁盐
 
Immobilized Cell Production Of Acetic Acid Bacteria Optimization

Abstract: This paper is based on alginate as a carrier of fixed acetic acid fermentation ethanol production of acetic acid. Study of the immobilized cells Preparation, preparation of particle radius and the size. Also, the ring vaccination, seed cultivation time for the optimization. The most important thing is the alcohol of different initial concentrations of acid production, cell number, cell growth cycle, glucose consumption, the effects of ethanol consumption. Finally, we get the following conclusions : (1) a fixed cell average particle diameter of 5.0mm, particle size of 65.42mm3; (2) optimize the number of vaccines in 15 Central, Training time for between 36-48 hours, the number of bacteria broth up; (3) The concentration of glucose and glucose OD value of the conversion formula : y = 4.7237x, unit of mg/ml; (4) the number of bacteria cells OD value of the correlation : y = 402.88x, 107 units/ml; (5) the initial alcohol concentration of acetic acid production : in the alcohol concentration. 5% alcohol concentration of acetic acid fermentation broth highest conversion rate, the best time to train 96 hours; (6) the initial alcohol concentration of cell number : The impact of different initial concentrations of alcohol affects cell growth, The higher the alcohol concentration in the cell growth plays an extended period of growth delay; (7) the initial alcohol concentration of glucose consumption : The impact of the higher ethanol content of the glucose consumption is smaller; (8) the initial alcohol concentration of ethanol consumption : The impact of the 72-hour time point, ethanol consumption has been completed, acetate in 72 -96 hours of the continued generation, Acetaldehyde is not with the consumption of alcohol finished immediately into acetic acid resulting from the lagging response
 Keywords：Immobilized；Ethanol；Acetic acid bacteria；Calcium magnesium acetate

目  录
中文摘要┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉Ⅰ
英文摘要┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉Ⅱ
目录┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉Ⅲ
1.绪论┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉1
 1.1 除冰剂的使用概况┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉1
 1.2 用海藻酸钠固定化醋酸菌┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉4
 1.3 固定化细胞的特性┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉4
 1.4 醋酸菌的醋酸发酵原理┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉5
 1.5发酵培养中醋酸细胞的生长状况 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉6
2．材料和方法 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉7
 2.1材料 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉7
 2.2 实验方法与制备过程 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉8
 2.3 分析步骤 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉8
3. 结果和讨论  ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉10
 3.1 接种环数优化 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉10
 3.2 葡萄糖标准曲线制作 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉12
 3.3 菌种OD值相关性研究┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉13
 3.4 酒精初始浓度对乙酸产量的影响研究 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉14
 3.5酒精初始浓度对菌体个数的影响研究┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉16
 3.6酒精初始浓度对葡萄糖消耗的影响研究┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉17
 3.7 酒精初始浓度对乙醇消耗的影响研究 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉19
 3.8 小结 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉20
4. 总结┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉21
致谢 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉22
参考文献 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉23

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