牛乳中假单胞菌属的PCR快速检测

文档编号:SW113  文档字数:10627,页数:29

摘 要：本文主要介绍了牛乳中假单胞菌属的鉴定和PCR快速检测。牛乳在储存过程中，大多数是嗜冷菌，主要是假单胞菌属和一些杆菌属等，会产生耐受巴氏消毒甚至UHT处理的蛋白酶和脂肪酶，分解牛乳中的蛋白质和脂肪。分解产物将导致乳制品风味改变，品质降低，保质期缩短，对产品的形象和消费者的健康都造成了最大的危害。为了探索对这一问题的有效解决方法，本文对低温贮藏原料乳中的嗜冷菌进行了研究。通过生理生化试验，初步确定其所在的菌属，利用通用PCR通用引物，提取假单胞菌属的基因组DNA进行PCR快速检测，最后进行测序比对确定其具体菌属。
关键词：假单胞菌属：生理生化试验：DNA提取；PCR
PCR For Rapid detection of Pseudomonas in milk

Abstract: This article is about PCR For Rapid detection of Pseudomonas in milk. Microorganism contamination of dairy products has been a key factor in intense market competition. When lac vaccinum is in reserve, most psychrophilic bacteria, mainly Pseudomonas and Xanthomonas, produce proteinase and lipase which tolerate pasteurization even UHT curing, reducing protein and grease. Cleavage products will change special flavor, deteriorate quality, shorten deadline, heavily influence the quality of dairy products, which will damage the products image and consumer health. In order to research the method to solve the problems, the paper studied the psychrophilic bacteria in the raw milk preserved in low temperature. This study discusses the PCR detection of  Pseudomonas bacteria , and the using the bacteria common PCR primer, collected genome DNA for PCR rapid test, tested and contrasted sequence ,the confirmed the bacterium.
Keywords：Pseudomonas ; Physiological and Biochemical；DNA extraction ；PCR

目  录
中文摘要 I
英文摘要 II
目录 Ⅲ
1 绪论 1
1.1 假单胞菌属简介 1
1.2 分子生物学研究进展 3
1.3 立题的根据与意义 5
2 实验内容 7
2.1 生理生化实验 7
2.2 DNA快速提取 9
2.3 牛乳中假单胞菌属的PCR快速检测 11
3 实验结果与讨论 14
3.1 生理生化试验结果 14
3.2 提取DNA实验现象 15
3.3 PCR快速检测、DNA回收及测序比对结果 16
3.4 实验结果与分析 20
3.4.1 生理生化试验 20
4 总结与展望 22
致谢 23
参考文献 24

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